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Sequencing Reaction with Big Dye v3.0
Standard
Standard
1/16
1/8
H20
5.625
5.125
5X
1.75
1.75
BigDye
0.5
1
Primer (100 μm)
0.125
0.125
DNA (10-75ng)
2
2
Total volume
10
10
Kelly B. Miller Lab, Department of Biology, University of New Mexico, 167 Castetter Hall, MSC03 2020, Albuquerque, NM 87131-0001 USA
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